Facilities

NMR spectrometers at MLU are operated in three locations. The locations are assigned to the participating research institutes, but are within walking distance of each other. At the Institute of Physics, two research groups work with their respective devices in the same building, which was specially built for the NMR spectrometers, but are listed separately.
The following is a list of the different locations with their equipment and measurement methods.

Location NF2C at the Institute of Chemistry
Spectrometers

500MHz

Console:
• Agilent DD2

Probe:
• 5 mm PFG OneNMR 1H(19F), 15N-31P, Z-Grad.

400MHz

Console:
• Agilent VNMRS

Probe:
• 5 mm PFG OneNMR 1H(19F), 15N-31P, Z-Grad.

2 x Benchtop 80MHz

Consoles:
• Magritek Spinsolve
• Bruker Fourier80

Probes:
• 5 mm; 1H, 13C, 19F; autosampler, flow cell
• 5 mm; 1 H, 31P

Methods
    • All common high-resolution 1H- and X-nucleus 1D and 2D characterisation methods, e.g. DEPT/APT, COSY, NOESY, HSQC/HETCOR…

Location NF1BC at the Institute of Biochemistry
Spectrometers

700MHz

Console:
• Bruker Avance III

Probes:
• 5 mm TXI 1H, 13C, 15N, Z-Grad.;
• 5 mm BBI 1H, 31P-207Pb, Z-Grad.;
• 4 mm HR-MAS DVT 1H,13C,15N, 2H, Z-Grad., 6 kHz

400MHz

Console:
• Bruker Avance III

Probes:
• 5 mm TBI 1H, 13C, 31P – 15N Z-Grad.;
• 5 mm BBFO 1H(19F), 31P-109Ag, Z-Grad.;
• 4 mm HR-MAS 1H, 13C, 15N, 2H Z-Grad. 6 kHz;
• 4 mm HR-MAS 1H, 31P, 13C, 2H Z-Grad. 6 kHz

Benchtop 60MHz

Console:
• Magritek Spinsolve

Probe:
• 1H, 19F, 13C

Methods
    • All common high-resolution 1H/19F- and X-nucleus (X = 13C, 15N, 31P, 33S, 67Zn…) 1D and 2D characterisation methods, e.g. DEPT/APT, COSY, NOESY, ROESY, TOCSY, HSQC, HMBC, DOSY, etc.
    • HR-MAS rotor systems 4 mm (up to 6 kHz) with 2H-lock and 1H/13C/15N or 1H/31P/13C channels for the investigation of highly viscous samples
    • 13C, 15N relaxometry (T1, T1ρ, T2, HetNOE) for the investigation of molecular dynamics

Due to the limited sample throughput resulting from the mixed operation, the service offer concentrates on more elaborate measurement series on selected samples.

Location NF2P at the Institute of Physics – NMR
Spectrometers

3 x 400MHz (wide bore)

Consoles:
• Bruker Avance III
• Bruker Avance II/III

Probes:
• 4 mm MAS DVT BB HXY
• 4 mm MAS WVT BB HX
• 3.2 mm MAS DVT BB HX
• 5 mm static VTN BB HX with goniometer option
• 5 mm Diff60/Micro5, 1H, X (inserts: 2H, 13C, 15N, 7Li, 19F)

200MHz (wide bore)

Console:
• Bruker Avance III

Probe:
• 5 mm static VTN 1H

3 x Minispec 20MHz

Console:
• Bruker Minispec mq20

Probes:
• 1H low field relaxometry
• 1 device with pulsed field gradient diffusometry

Methods
    • 1H/19F- and 1H-X (X=13C, 15N, 31P, 2H, 29Si, 6/7Li, 11B, …) CP or DP MAS NMR, 1D, 2D HETCOR/WISE, DQ, etc.,
    • MAS rotor systems 4 mm (up to 15 kHz), 3.2 mm (up to 24 kHz), 2.5 mm (up to 35 kHz) and 1.3 mm (up to 70 kHz, especially for 1H high-resolution in solids), 3.2 mm MAS E-free system (600 MHz) for aqueous/conductive samples
    • Static broad line spectroscopy (1H, 2H, 13C, 31P, …) for dynamic measurements
    • 1H-13C order parameter measurements (DIPSHIFT/PDLF), especially for membrane lipids and liquid crystals
    • 1H and X-nucleus relaxometry (T1, T1ρ, T2), high resolution (MAS) or in the time domain, for characterisation of molecular dynamics, dynamic heterogeneities and multiphase structure
    • Static 1H multiquantum NMR (especially low-field/benchtop) to study chain dynamics and crosslinking structure in polymer melts, elastomers and gels
    • Static and MAS (CODEX) exchange NMR for slow dynamics
    • Pulsed field gradient diffusometry (up to 30 T/m) for slow diffusion in soft matter up to D ~ 5 x 10-15 m2/s
    • Biomolecular solid-state NMR, especially high-resolution 15N- and 1H-based relaxometry for protein dynamics

Location NF2P at the Institute of Physics – Biophysics
Spectrometers

800MHz

Console:
• Bruker Avance III

Probes:
• 5 mm TXO Cryo 13C observe, 1H, 15N, Z-Grad.
• 5 mm TCI Cryo 1H, 13C, 15N, Z-Grad.
• 5 mm QXI 1H, 31P, 13C, 15N, Z-Grad.

2 x 600MHz

Consoles:
• Bruker Avance III
•Bruker Avance II+

Probes:
• 5mm TXI 1H, 13C, 15N, Z-Grad.
• 3 mm TBI 1H, 13C, 31P – 15N, Z-Grad.
• 5 mm QXI 1H, 31P, 13C, 15N, Z-Grad.
• 8 mm mixing chamber probe 1H, 13C, Z-Grad.
• 5 mm TXI 1H, 13C, 15N, Z-Grad.
• 5mm BBI 1H, 31P-109Ag, Z-Grad.
• 4 mm MAS DVT 1H, 13C, 15N, 15 kHz
• 2.5 mm MAS VTN 1H, 15N-31P, 35 kHz high pressure setup up to 2500 bar

500MHz

Console:
• Bruker AMX/DRX

Probe:
• 5 mm TXI 1H, 13C, 15N Z-Grad.

Methods
    • Multi-dimensional multiresonance experiments on isotope-marked proteins (15N, 13C, 2H)
    • Time-resolved protein NMR
    • Relaxation dispersion
    • High pressure NMR up to 2500bar